JoVE Logo
Faculty Resource Center

Sign In

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo (Video) | JoVE

DOI :

10.3791/58503-v

June 28th, 2019

June 28th, 2019

6,846 Views

1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health

Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.

-- Views

Related Videos

article

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

article

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

article

A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat

article

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

article

A Novel and Translational Rat Model of Concussion Combining Force and Rotation with In Vivo Cerebral Microdialysis (Video) | JoVE

article

Quantitative Measurement of Intrathecally Synthesized Proteins in Mice (Video) | JoVE

article

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans (Video) | JoVE

article

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue (Video) | JoVE

article

Cerebellar Regional Dissection for Molecular Analysis (Video) | JoVE

article

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved