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Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

DOI :

10.3791/58503-v

June 28th, 2019

June 28th, 2019

6,816 Views

1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health

Protein synthesis is a critical biological process for cells. In brain, it is required for adaptive changes. Measurement of rates of protein synthesis in the intact brain requires careful methodological considerations. Here we present the L-[1-14C]-leucine quantitative autoradiographic method for determination of regional rates of cerebral protein synthesis in vivo.

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