May 19th, 2022
•The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.
Related Videos
Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
Double Fluorescence in situ Hybridization in Fresh Brain Sections
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures
Morphometric Analyses of Retinal Sections
Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
Quantification of Immunostained Caspase-9 in Retinal Tissue
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved