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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

DOI :

10.3791/58621-v

January 7th, 2019

January 7th, 2019

10,005 Views

1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

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