JoVE Logo
Faculty Resource Center

Sign In

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells (Video) | JoVE

DOI :

10.3791/58621-v

January 7th, 2019

January 7th, 2019

10,102 Views

1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

-- Views

Related Videos

article

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

article

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

article

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

article

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

article

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells (Video) | JoVE

article

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow (Video) | JoVE

article

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis (Video) | JoVE

article

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice (Video) | JoVE

article

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes (Video) | JoVE

article

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved