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Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

DOI :

10.3791/58640-v

November 17th, 2018

November 17th, 2018

11,137 Views

1Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine, Johns Hopkins University, 3Institute for Nanobiotechnology, Johns Hopkins University, 4Department of Pathology, School of Medicine, Johns Hopkins University

Antigen-specific T cells are difficult to characterize or utilize in therapies due to their extremely low frequency. Herein, we provide a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells and then to expand them several hundred-fold for both characterization and therapy.

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