Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4⁺ T-cell Immunophenotyping and HIV Reservoir Assessment

20.7K Views

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07:21 min

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June 23rd, 2019

DOI :

10.3791/59427-v

June 23rd, 2019

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Syim Salahuddin*¹^,², Elaine Thomson*¹^,²^,³, Oussama Méziane¹^,², Omar Farnos², Amélie Pagliuzza⁵, Nicolas Chomont⁵^,⁶, Ron Olivenstein¹, Cecilia Costiniuk¹^,³^,⁴, Mohammad-Ali Jenabian²^,³^,⁶

¹Research Institute McGill University Health Centre, ²Department of Biological Sciences, Université de Québec à Montréal, ³Department of Microbiology & Immunology, McGill University, ⁴Department of Medicine, Division of Infectious Diseases, McGill University, ⁵Centre de Recherche du Centre Hospitalier de l'Université de Montréal, ⁶Département de microbiologie, infectiologie et immunologie, Université de Montréal

Summary

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Bronchoalveolar Lavage

Pulmonary Mucosal T Cells

Primary Macrophages

BAL Fluid Processing

Virologic Assessment

Inflammatory Diseases

Centrifugation

RPMI 1640 Medium

Fetal Bovine Serum

Peripheral Blood Mononuclear Cells

Cell Sorting

Chapters in this video

0:04

Title

1:02

Bronchoalveolar (BAL) Cell Isolation

2:33

Whole BAL Cell and Peripheral Blood Mononuclear Cell (PBMC) Sorting

5:48

Results: Representative Alveolar Macrophage Immunophenotyping

6:41

Conclusion

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Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment

Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4⁺ T-cell Immunophenotyping and HIV Reservoir Assessment