Assembly of the Modified Parallel Plate Flow Chamber
1:43
Cell Preparation and Seeding into the MPP Flow Chamber
3:43
Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
5:46
Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
6:43
Conclusion
Transcript
Use of the modified parallel plate flow chamber can help researchers answer key questions pertaining to the functional response of mechanosensitive ion channels to shear stress. The main advantage of this technique is that it allows real-time inve
Sign in or start your free trial to access this content
The goal of this protocol is to describe a modified parallel plate flow chamber for use in investigating real time activation of mechanosensitive ion channels by shear stress.