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JoVE Journal

Developmental Biology

This content is Open Access.

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Transcript

This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.

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