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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

DOI :

10.3791/59980-v

October 15th, 2019

October 15th, 2019

26,188 Views

1Department of Pathology, University of Iowa, 2Medical Scientist Training Program, University of Iowa, 3Graduate Program in Immunology, University of Iowa, 4Graduate Program in Molecular Medicine, University of Iowa

Described here is a simplified standard operating procedure for microbiome profiling using 16S rRNA metagenomic sequencing and analysis using freely available tools. This protocol will help researchers who are new to the microbiome field as well as those requiring updates on methods to achieve bacterial profiling at a higher resolution.

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Microbiota Analysis

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