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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

DOI :

10.3791/58481-v

8:33 min

November 17th, 2018

November 17th, 2018

12,109 Views

1The Peter Gorer Department of Immunobiology, King's College London, 2The Francis Crick Institute

Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

Tags

Quantitative Polymerase Chain Reaction based Analyses

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