JoVE Logo
Faculty Resource Center

Sign In

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

DOI :

10.3791/58481-v

8:33 min

November 17th, 2018

November 17th, 2018

11,950 Views

1The Peter Gorer Department of Immunobiology, King's College London, 2The Francis Crick Institute

Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

Tags

Quantitative Polymerase Chain Reaction based Analyses

-- Views

Related Videos

article

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

article

Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis

article

Murine Fecal Isolation and Microbiota Transplantation

article

Whole-Mount Fluorescence In Situ Hybridization to Study Spermatogenesis in the Anopheles Mosquito

article

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

article

Author Spotlight: Detection and Treatment of Helicobacter pylori Infection

article

Author Spotlight: Neutrophil Extracellular Traps Imaging in Human and Mouse Tissues

article

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

article

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis

article

Author Spotlight: Studying Macrophage-Epithelial Cell Interactions in Salivary Gland Regeneration After Injury

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved