JoVE Logo
Faculty Resource Center

Sign In

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

DOI :

10.3791/58239-v

August 25th, 2018

August 25th, 2018

12,267 Views

1Department of Biology, San Francisco State University

Here we present a next-generation sequencing protocol for 16S rRNA sequencing which enables identification and characterization of microbial communities within vectors. This method involves DNA extraction, amplification and barcoding of samples through PCR, sequencing on a flow-cell, and bioinformatics to match sequence data to phylogenetic information.

-- Views

Related Videos

article

Experimental Approach for Determining Semiconductor/liquid Junction Energetics by Operando Ambient-Pressure X-ray Photoelectron Spectroscopy

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

article

Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

article

Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation

article

Author Spotlight: Decoding Corneal Neovascularization with Alkali Burn Model for Future Therapeutic Strategies

article

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

article

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

article

Author Spotlight: Unraveling the Pathogenesis of Age-Related Macular Degeneration and Discovering Potential Therapies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved