Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
8.1K Views
•
10:04 min
•
September 28th, 2019
DOI :
September 28th, 2019
•Jonathon M. Muncie1,2, Roberto Falcón-Banchs1, Johnathon N. Lakins2, Lydia L. Sohn1,3, Valerie M. Weaver2,4,5,6
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, 2Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California San Francisco, 3Department of Mechanical Engineering, University of California Berkeley, 4Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 5UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center, University of California San Francisco, 6Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology, University of California San Francisco
Chapters in this video
0:04
Title
0:49
Generation of Stencils for Patterning ECM Ligand
3:47
Patterning ECM Ligand on Coverslips
4:46
Transfer of Ligand to Polyacrylamide Gel
7:27
Results: Geometry of Human Embryonic Stem Cell Colonies
9:04
Conclusion
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