Microinjectrode System for Combined Drug Infusion and Electrophysiology

6.7K Views

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08:30 min

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November 13th, 2019

DOI :

10.3791/60365-v

November 13th, 2019

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M. Isabel Vanegas¹, Kenneth R. Hubbard¹^,², Rahim Esfandyarpour³^,⁴, Behrad Noudoost¹

¹Department of Ophthalmology and Visual Sciences, University of Utah, ²Department of Biomedical Engineering, University of Utah, ³Department of Electrical Engineering and Computer Science, University of California, Irvine, ⁴Department of Biomedical Engineering, University of California, Irvine

Summary

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Microinjectrode System

Microfluidic Infusion

Awake Behaving Animals

Permeability

Cannula Configuration

Nanoliter Scale

T Junction Assembly

Gold Pin Soldering

Epoxy Glue Attachment

Microfluidic Circuit Construction

Chapters in this video

0:04

Title

0:46

Construction of the Microinjectrode for Stimulation and Recording

2:21

Microfluidic System

3:22

Preparation of the Microfluidic System

5:46

Performing Recording for an Infusion Experiment

6:38

Results: Memory Guided Saccade Task during Muscimol Infusion in FEF (Frontal Eye Field)

7:26

Conclusion

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