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Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

DOI :

10.3791/53058-v

11:08 min

September 5th, 2015

September 5th, 2015

13,016 Views

1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Departments of Physiology and Neuroscience, New York University School of Medicine

We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.

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Double barreled Microelectrodes

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