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Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

DOI :

10.3791/60646-v

July 25th, 2020

July 25th, 2020

6,928 Views

1Center for Individualized Medicine, Mayo Clinic, 2Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic

Here we present a protocol to test the efficacy of targeted therapies selected based on the genomic makeup of a tumor. The protocol describes identification and validation of structural DNA rearrangements, engraftment of patients’ tumors into mice and testing responses to corresponding drugs.

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