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Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies

DOI :

10.3791/54944-v

November 30th, 2016

November 30th, 2016

11,744 Views

1Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus

We describe a method for stable labeling of patient-derived xenografts (PDXs) with lentiviral particles expressing green-fluorescent protein and luciferase reporters. This method allows for tracking the growth of PDXs at the primary site, as well as detecting spontaneous and experimental metastases using in vivo imaging systems.

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