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PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

DOI :

10.3791/60661-v

15:04 min

November 2nd, 2019

November 2nd, 2019

10,217 Views

1Department of Biochemistry, University of British Columbia, 2School of Biochemistry, University of Bristol

We present a method that combines membrane protein purification and reconstitution into peptidiscs in a single chromatographic step. Biotinylated scaffolds are used for direct surface attachment and measurement of protein-ligand interactions via biolayer interferometry.

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