The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
9.5K Views
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11:45 min
•
February 27th, 2020
DOI :
February 27th, 2020
•1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University
Chapters in this video
0:05
Title
1:36
Transfection, Chemical Fixation and Peroxidase Reaction
3:48
High Pressure Freezing
4:46
Freeze Substitution
6:23
Resin Infiltration and Embedding
8:11
Sectioning and TEM Imaging
9:17
Results: TEM Images
11:07
Conclusion
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