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Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

DOI :

10.3791/54066-v

June 23rd, 2016

June 23rd, 2016

10,844 Views

1Department of Biological Chemistry, Weizmann Institute of Science, 2Department of Chemical Research Support, Weizmann Institute of Science, 3Institute of Plant Sciences, Agricultural Research Organization, Volcani Center

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.

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