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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

DOI :

10.3791/55664-v

10:01 min

May 1st, 2017

May 1st, 2017

13,315 Views

1Department of Cell Biology, Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine

We developed a novel technique in electron microscopy, "flash-and-freeze," that enables the visualization of membrane dynamics with ms temporal resolution. This technique combines the optogenetic stimulation of neurons with high-pressure freezing. Here, we demonstrate the procedures and describe the protocols in detail.

Tags

Membrane Dynamics

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