Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
11.9K Views
•
09:03 min
•
March 10th, 2020
DOI :
March 10th, 2020
•Chapters in this video
0:05
Introduction
0:43
Primary Screening: Poly-L-Lysine Plate Preparation
1:25
Primary Screening: Cell Culture and Plate Seeding
2:22
Primary Screening: DNA Plate Preparation and Transfections
3:35
Primary Screening: Cell Stimulation and Luminescence Reading
4:26
Secondary Screening: Cell Seeding and Transfections
5:59
Secondary Screening: Half Log Dose-Curve Drug Plate Preparation and Luminescence Reading
7:16
Results: Representative Primary Screening Hit Identification and Secondary Screening Dose-Curve Responses
8:23
Conclusion
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