Primary Screening: DNA Plate Preparation and Transfections
3:35
Primary Screening: Cell Stimulation and Luminescence Reading
4:26
Secondary Screening: Cell Seeding and Transfections
5:59
Secondary Screening: Half Log Dose-Curve Drug Plate Preparation and Luminescence Reading
7:16
Results: Representative Primary Screening Hit Identification and Secondary Screening Dose-Curve Responses
8:23
Conclusion
Transcription
The Presto-Tango platform was devised to execute the parallel and simultaneous interrogation of all non-olfactory GPCRs in the human genome, including that orphan targets to profile ligand-induced beta-arrestin 2 recruitment. The Presto-Tango plat
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Given that GPCRs are attractive druggable targets, GPCR ligand screening is thus indispensable for the identification of lead compounds and for deorphanization studies. Towards these efforts, we describe PRESTO-Tango, an open-source resource platform used for simultaneous profiling of transient β-arrestin2 recruitment at approximately 300 GPCRs using a TEV-based reporter assay.