June 7th, 2020
•We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.
Tags
Related Videos
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
An In Vivo Estrogen Deficiency Mouse Model for Screening Exogenous Estrogen Treatments of Cardiovascular Dysfunction After Menopause
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved