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In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

DOI :

10.3791/61037-v

May 1st, 2020

May 1st, 2020

6,374 Views

1Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences, Singapore Polytechnic, 3Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)

This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.

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In Vitro Model

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