JoVE Logo
Faculty Resource Center

Sign In

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

DOI :

10.3791/3227-v

8:01 min

December 22nd, 2011

December 22nd, 2011

57,116 Views

1Graduate Group in Biophysics, University of California San Francisco , 2Department of Biochemistry and Biophysics, University of California San Francisco

Visualizing protein samples by negative stain electron microscopy (EM) has become a popular structural analysis method. It is useful for quantitative structural analysis, such as calculating a 3D reconstruction of the molecules being studied, and also for qualitative examination of the quality of protein preparations. In this article we present detailed protocols for preparing the EM grids, staining the sample and visualizing the sample in an electron microscope. Novice users can follow these protocols easily and to utilize negative stain EM as a routine assay, in addition to other biochemical assays, for evaluating their protein samples.

Tags

Visualizing

-- Views

Related Videos

article

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

article

Cell Co-culture Patterning Using Aqueous Two-phase Systems

article

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

article

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

article

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

article

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

article

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

article

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

article

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

article

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved