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A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

DOI :

10.3791/61081-v

June 12th, 2020

June 12th, 2020

4,888 Views

1Department of Biology, The University of Akron, 2Department of Geosciences, The University of Akron

We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

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Sectioning

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