August 5th, 2021
•Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.
Related Videos
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
Single Particle Cryo-Electron Microscopy: From Sample to Structure
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids (Video) | JoVE
Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques
Optimizing Grid Preparation for Enhanced Cryoelectron Tomography (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved