Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

7.0K Views

•

07:31 min

•

May 7th, 2020

DOI :

10.3791/61085-v

May 7th, 2020

•

Larissa Kraus¹, Laura Monni¹^,², Ulf C. Schneider³, Julia Onken³, Philipp Spindler³, Martin Holtkamp¹, Pawel Fidzinski¹^,²

¹Department of Neurology with Experimental Neurology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ³Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Summary

Explore More Videos

Acute Human Hippocampal Slices

Electrophysiological Recordings

Anti epileptic Substances

Temporal Lobe Epilepsy

Human Brain Tissue

Choline ACSF Solution

Double distilled Water

Osmolarity Measurement

Carbogen Gas

Slice holding Compartment

Vibratome Preparation

Tissue Slicing Procedure

Chapters in this video

0:04

Introduction

0:33

Solution Preparation

2:01

Interface Chamber Preparation

2:39

Preparation Area Setup

3:39

Tissue Slicing and Storing

4:37

Epileptiform Activity Recording

5:59

Results: Representative Epileptiform and Epileptic Burst Activity

6:38

Conclusion

Related Videos

04:41

Preparation of Oligomeric β-amyloid_1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

23.1K Views

09:10

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

13.1K Views

16:38

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

27.2K Views

09:39

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

26.7K Views

09:23

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

14.6K Views

10:24

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

17.1K Views

11:29

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

13.8K Views

10:53

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

45.6K Views

07:02

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

9.9K Views

08:24

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

11.3K Views