May 16th, 2020
•This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.
Tags
Related Videos
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved