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JoVE Journal

Immunology and Infection

This content is Open Access.

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

3.8K Views

09:42 min

May 16th, 2020

May 16th, 2020

3,817 Views

0:04

Introduction

0:57

Zebrafish Larva Hindbrain Ventricle Microinjection

4:21

Establishment of Injected and Viable Spore Numbers

5:22

Drug Treatment of Larvae and Survival Analysis

5:53

Zebrafish Wounding and Entrapment Device for Growth and Imaging

7:23

Results: Infection Outcome of Aspergillus Spores Microinjected into Zebrafish Larvae

9:07

Conclusion

Transcript

This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.

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