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Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices

9.8K Views

11:19 min

May 10th, 2020

May 10th, 2020

9,848 Views

0:04

Introduction

0:41

Preparing the Cardiac Tissue

2:27

Using the Vibratome

4:00

Tissue Digestion and Dissociation

7:03

Removing Mechanical Uncoupling Agent

8:08

Results: Vibratome-Cut Slices Yield Viable Cardiomyocytes

10:33

Conclusion

Transcript

This protocol works with samples from human and animal hearts, even after hours of transportation time. Up to 200 myocytes per milligram can be isolated from vibratome-cut tissue slices. Electrophysiological, structural and biochemical analysis of

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Presented is a protocol for the isolation of human and animal ventricular cardiomyocytes from vibratome-cut myocardial slices. High yields of calcium-tolerant cells (up to 200 cells/mg) can be obtained from small amounts of tissue (<50 mg). The protocol is applicable to myocardium exposed to cold ischemia for up to 36 h.

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