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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

DOI :

10.3791/51255-v

9:13 min

April 30th, 2014

April 30th, 2014

11,855 Views

1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.

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In Vivo

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