Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

4.3K Views

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08:42 min

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July 3rd, 2020

DOI :

10.3791/61390-v

July 3rd, 2020

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Yiling Yang*¹, Qianye Chen*², Siru Zhou¹, Xinyi Gong¹, Hongyuan Xu¹, Yueyang Hong¹, Qinggang Dai³, Lingyong Jiang¹

¹Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, ²Department of Stomatology, Dalian Medical University, ³The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology

Summary

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Stat3 Deletion

Mouse Model

Skeletal Phenotype Analysis

Genetically Engineered Mice

Skeletal Tissue Research

Alizarin Red Labeling

Decalcification Process

Embedding Specimens

Chapters in this video

0:04

Introduction

0:54

Specimen Collection

2:13

Paraffin Section Preparation

3:34

TRAP Staining

5:34

Calcein and Alizarin Red Double Labeling

6:55

Results: Effects of Stat3 Deletion on Osteoclast Differentiation

8:09

Conclusion

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