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Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

DOI :

10.3791/61481-v

6:54 min

September 9th, 2020

September 9th, 2020

5,648 Views

1Department of Neuroscience and Experimental Therapeutics, Texas A&M University Health Science Center, College of Medicine, 2Texas A&M Institute for Neuroscience

Here we present a protocol to measure in vitro Ca2+ fluxes in midbrain neurons and their downstream effects on caspase-3 using primary mouse midbrain cultures. This model can be employed to study pathophysiologic changes related to abnormal Ca2+ activity in midbrain neurons, and to screen novel therapeutics for anti-apoptotic properties.

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