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Measuring Fast Calcium Fluxes in Cardiomyocytes

DOI :

10.3791/3505-v

November 29th, 2011

November 29th, 2011

14,935 Views

1Department of Biological Sciences and Geology, Queensborough Community College, 2Department of Physiology & Biophysics, Stony Brook University

We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.

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Calcium Fluxes

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