JoVE Logo
Faculty Resource Center

Sign In

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

DOI :

10.3791/61483-v

8:37 min

September 9th, 2020

September 9th, 2020

4,876 Views

1Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias, Universidad Autónoma del Estado de México

This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.

Tags

Immunohistochemistry Techniques

-- Views

Related Videos

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

article

Assembly and Operation of a Cooling Stage to Immobilize C. elegans on Their Culture Plates

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Direct Infusion Device for Molecule Delivery in Plants

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved