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Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

DOI :

10.3791/61483-v

8:37 min

September 9th, 2020

September 9th, 2020

5,268 Views

1Laboratorio de Neurociencias, Departamento de Psicología, Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias, Universidad Autónoma del Estado de México

This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.

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Immunohistochemistry Techniques

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