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Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

DOI :

10.3791/52353-v

March 4th, 2015

March 4th, 2015

11,972 Views

1Department of Physiology, University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, 3Department of Education and Research, Taipei City Hospital

Here we describe a technique for studying hippocampal postnatal neurogenesis using the organotypic slice culture technique. This method allows for in vitro manipulation of adult neurogenesis and allows for the direct application of pharmacological agents to the cultured hippocampus.

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