June 2nd, 2020
•This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS).
Related Videos
Obtaining Eggs from Xenopus laevis Females
Preparation and Fractionation of Xenopus laevis Egg Extracts
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
Transplantation of GFP-expressing Blastomeres for Live Imaging of Retinal and Brain Development in Chimeric Zebrafish Embryos
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved