JoVE Logo

Sign In

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

5.2K Views

07:38 min

September 1st, 2020

DOI :

10.3791/61580-v

September 1st, 2020


Explore More Videos

Fast Photochemical Oxidation

Chapters in this video

0:04

Introduction

0:47

Fast Photochemical Oxidations of Proteins (FPOP) Optical Bench Preparation

2:43

FPOP Experiment

3:58

Compensation Performance

5:45

Results: Representative Adalimumab FPOP and Compensated Myoglobin FPOP in MES Buffer

6:51

Conclusion

Related Videos

article

10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

17.9K Views

article

08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

11.2K Views

article

16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

17.7K Views

article

09:03

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

5.5K Views

article

09:59

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

3.6K Views

article

05:50

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

3.2K Views

article

08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

8.5K Views

article

14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

5.0K Views

article

07:09

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

2.4K Views

article

08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

2.4K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved