JoVE Logo

Sign In

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

3.6K Views

09:59 min

June 4th, 2021

DOI :

10.3791/61861-v

June 4th, 2021


Explore More Videos

Hydroxyl Radical Protein Footprinting

Chapters in this video

0:04

Introduction

0:35

Installing the Capillary Tube

2:43

Installing an Injection Loop

3:34

Initializing the Laser Free HRPF System

4:45

Modification of Protein to Detect Changes in Higher Order Structure

6:59

Shutting Down the Laser Free HRPF System

7:43

Results: High Order Structural Analysis of Proteins Using Laser-free Hydroxyl Radical Protein Footprinting

9:15

Conclusion

Related Videos

article

07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

80.4K Views

article

11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

18.3K Views

article

08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

6.1K Views

article

08:00

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

6.8K Views

article

09:03

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

5.5K Views

article

07:38

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

5.2K Views

article

05:50

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

3.2K Views

article

10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

5.3K Views

article

10:59

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

8.5K Views

article

13:41

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

14.2K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved