June 4th, 2021
•This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.
Tags
Related Videos
Combining Wet and Dry Lab Techniques to Guide the Crystallization of Large Coiled-coil Containing Proteins
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved