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Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

DOI :

10.3791/61623-v

10:18 min

July 9th, 2020

July 9th, 2020

2,418 Views

1Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 2Center for Brain Function and Repair, Rosalind Franklin University of Medicine and Science

This protocol presents a method for imaging neuronal population activity with single-cell resolution in non-transgenic invertebrate species using absorbance voltage-sensitive dyes and a photodiode array. This approach enables a rapid workflow, wherein imaging and analysis can be pursued over the course of a single day.

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Photodiode based Optical Imaging

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