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Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

DOI :

10.3791/4261-v

November 29th, 2012

November 29th, 2012

16,435 Views

1Department of Cellular and Molecular Physiology, Yale University School of Medicine

An imaging technique for monitoring of membrane potential changes with sub-micrometer spatial and sub-millisecond temporal resolution is described. The technique, based on laser excitation of voltage-sensitive dyes, allows measurements of signals in axons and axon collaterals, terminal dendritic branches, and individual dendritic spines.

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