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Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

2.9K Views

08:29 min

September 9th, 2020

September 9th, 2020

2,891 Views

0:04

Introduction

0:39

Light Sheet Fluorescence Microscope Calibration

2:37

Sodium Hypochlorite-Based Dechorionation

3:48

Embryo Mounting

4:51

Comparative Live Imaging

6:02

Imaged Embryo Retrieval and Cultivation

6:52

Results: Representative Drosophila and Tribolium Fluorescence Live Imaging

7:56

Conclusion

Transcript

Sample Chamber-Based Light Sheet Florescence Microscopes are designed for high content rather than high throughput. Live imaging assays must therefore be performed sequentially and thus less affected by ambient variance. Our cobweb holder allow st

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Light sheet-based fluorescence microscopy is the most valuable tool in developmental biology. A major issue in comparative studies is ambient variance. Our protocol describes an experimental framework for simultaneous live imaging of multiple specimens and, therefore, addresses this issue pro-actively.

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