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Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

DOI :

10.3791/4305-v

December 13th, 2012

December 13th, 2012

11,593 Views

1Department of Oncology, University of Wisconsin - Madison

A method of observing individual DNA molecules in live cells is described. The technique is based on the binding of a fluorescently tagged lac repressor protein to binding sites engineered into the DNA of interest. This method can be adapted to follow many recombinant DNAs in live cells over time.

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Plasmid Replication

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