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A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

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14:34 min

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May 6th, 2022

DOI :

10.3791/61776-v

May 6th, 2022

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3,361 Views

Wei Zhang^1*, Xinjuan Lei^1*, Xin Zhou^2,3*, Boling He¹, Liqin Xiao¹, Huimin Yue¹, Shulin Wang¹, Yuting Sun¹, Yajun Wu¹, Liyang Wang^1,4, George Ghartey-Kwansah¹, Odell D. Jones⁵, Joseph L. Bryant⁶, MengMeng Xu⁷, Jianjie Ma³, Xuehon Xu¹

¹National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, ²Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, ³Ohio State University College of Medicine, ⁴Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, ⁵University of Pennsylvania ULAR, ⁶University of Maryland School of Medicine, ⁷Columbia University Medical Center

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Keywords Drosophila

Immunohistochemistry

Protein Localization

Developmental Biology

Gelatin Coating

Bleach Dechorionation

Methanol Precipitation

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