A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
3.6K Views
•
14:34 min
•
May 6th, 2022
DOI :
May 6th, 2022
•Wei Zhang*1, Xinjuan Lei*1, Xin Zhou*2,3, Boling He1, Liqin Xiao1, Huimin Yue1, Shulin Wang1, Yuting Sun1, Yajun Wu1, Liyang Wang1,4, George Ghartey-Kwansah1, Odell D. Jones5, Joseph L. Bryant6, MengMeng Xu7, Jianjie Ma3, Xuehon Xu1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
Chapters in this video
0:05
Introduction
0:44
Coating Slides
1:41
Embedding of Drosophila Embryos
4:25
Hematoxylin-Eosin Staining
5:25
Periodic Acid-Silver Methenamine Staining
6:28
Immunohistochemistry
8:15
In Situ Hybridization
12:17
Results
13:32
Discussion
Related Videos
14:21
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
12.8K Views
10:17
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
11.4K Views
10:10
Drosophila Larval NMJ Immunohistochemistry
15.5K Views
10:13
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
19.2K Views
12:31
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
23.2K Views
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
18.2K Views
08:13
RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs
12.6K Views
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
12.6K Views
08:49
Direct Cryosectioning of Drosophila Heads for Enhanced Brain Fluorescence Staining and Immunostaining
451 Views
08:37
Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes
4.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved