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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

DOI :

10.3791/61830-v

6:54 min

October 27th, 2020

October 27th, 2020

11,040 Views

1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

Tags

TGF beta Signaling

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