JoVE Logo
Faculty Resource Center

Sign In

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

DOI :

10.3791/59795-v

July 17th, 2019

July 17th, 2019

5,834 Views

1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, 2The Cancer and Stem Cell Program, Duke-NUS Medical School

In this article, we presented a set of practical and feasible methods for characterizing disease-related mutants of RAF family kinases, which include in vitro kinase assay, RAF co-activation assay, and complementary split luciferase assay.

Tags

Disease related Mutants

-- Views

Related Videos

article

A Mouse Model of Fatigue Induced by Peripheral Irradiation

article

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

article

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

article

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

article

Detection of Protease Activity by Fluorescent Peptide Zymography

article

A Practical Guide for the Production and PET/CT Imaging of 68Ga-DOTATATE for Neuroendocrine Tumors in Daily Clinical Practice

article

In Vitro Assay to Study Tumor-macrophage Interaction

article

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

article

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

article

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved