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Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

DOI :

10.3791/61903-v

10:10 min

December 1st, 2020

December 1st, 2020

4,150 Views

1Department of Molecular and Structural Biochemistry, North Carolina State University, 2Neutron Scattering Division, Oak Ridge National Laboratory

Neutron protein crystallography is a structural technique that permits the localization of hydrogen atoms, thereby providing important mechanistic details of protein function. We present here the workflow for mounting a protein crystal, neutron diffraction data collection, structure refinement and analysis of the neutron scattering length density maps.

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Neutron Crystallography

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