JoVE Logo
Faculty Resource Center

Sign In

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

DOI :

10.3791/62364-v

10:32 min

April 23rd, 2021

April 23rd, 2021

2,264 Views

1Diamond Light Source Ltd, Harwell Science & Innovation Campus

Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.

Tags

Sample Preparation

-- Views

Related Videos

article

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

article

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

article

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

article

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

article

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

article

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

article

Fixed Target Serial Data Collection at Diamond Light Source

article

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

article

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

article

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved