Amplification and Passaging of Human Nasal Epithelial (HNE) Cells
2:26
Cryopreservation of Primary Nasal Epithelial Cells
3:06
Thawing Frozen Amplified HNE Cells
4:38
Differentiation of HNE Cells at the Air-Liquid Interface
5:23
Results: Analysis of Electrophysiological Properties of Differentiated HNE Cells and CFTR-Related Chloride Secretion Correction
7:59
Conclusion
Transcript
This protocol highlights the key steps for amplifying, differentiating, and cryopreserving primary nasal epithelial cells. In our culture conditions, primary nasal epithelial cells mimic the lung epithelium. This allows patient derived cultures an
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Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.