Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

6.1K Views

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10:28 min

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April 3rd, 2021

DOI :

10.3791/61991-v

April 3rd, 2021

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Camila F. Almeida¹, Emma C. Frair¹, Nianyuan Huang¹, Reid Neinast², Kim L. McBride²^,³^,⁴^,⁶, Robert B. Weiss⁵, Kevin M. Flanigan¹^,⁶, Nicolas Wein¹^,⁶

¹Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, ²Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, ³The Heart Center, Nationwide Children’s Hospital, ⁴Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, ⁵Department of Human Genetics, The University of Utah School of Medicine, ⁶Department of Pediatrics, The Ohio State University

Summary

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Direct Reprogramming

Human Fibroblasts

Myoblasts

Neuromuscular Disorders

Fibroblast Growth Medium

Differentiation Medium

Myotubes

Viral Transduction

Puromycin Lentivirus

Chapters in this video

0:00

Introduction

0:36

Overview

1:23

Initiation of Demal Fibroblasts Culture

2:46

Establishment of FM Cell Lines Lentvirus-hTERT Transduction

3:57

Lentivirus-MyoD Transduction

4:54

Fibroblasts Transdifferentiation

6:11

Results

9:16

Conclusion

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